Loading... Please wait...

Serotonin Ultrasensitive ELISA Assay Kit

Hover over image to zoom

$530.00
SKU:
SEU39-K01

 Product Description

Package insert view pdf

 

 

Serotonin Ultrasensitive ELISA Assay Kit:
For Research Use Only
Size:  1x96 wells
Sensitivity:  0.39 pg/sample
Dynamic Range:  0.67 - 20 pg/sample
Incubation Time:  Overnight
Sample Type:  Biological Fluids   
Sample Size: 1 - 20 µL
Controls Included
Product Developed and Manufactured in Germany EA630/96


Intended Use
The Eagle Biosciences Serotonin Ultrasensitive ELISA Assay
Kit is intended for the quantitative determination of serotonin in biological fluids. The Serotonin Ultrasensitive ELISA Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures. 
 
Assay Principle
The competitive Serotonin Ultrasensitive
ELISA Assay kit uses the microtiter plate format. Derivated serotonin competes for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase serotonin is detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase serotonin is inversely proportional to the serotonin concentration of the sample.

Assay Background

The Eagle Biosciences Serotonin Ultrasensitive ELISA Assay Kit is intended for the quantitative determination of serotonin in biological fluids. The Serotonin Ultrasensitive ELISA Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.  Serotonin (5-Hydroxytryptamine), a biogenic amine, is a product of the tryptophan metabolism. It is a well evaluated neurotransmitter of the central nervous system and can be found in high concentrations in the chromaffine cells of the intestinal mucosa, in the platelets and the serotonergic neurones of the brain.  Central-serotonergic neurones influence physiological functions such as sleep and the hormonal and cardio-vascular regulation. Increased serum levels can be found with malignant carcinoid, endogenous depression and schizophrenia.

The Serotonin Ultrasensitive ELISA Assay Kit provides materials for the quantitative measurement of derivated serotonin in low concentrated samples and for small sample volumes. The derivation is performed during the preparation of the samples. By using the acylation reagent, the serotonin is quantitatively derivated into N-acylserotonin.


Typical Standard Curve

Cross Reactivity Data

 

Substance

ED-50-Value (ng/ml)

Cross Reactivity (%)

Serotonin

4.3

100

Tryptamine

1,996

0.22

5-Methoxytryptamine

17,083

0.025

5-Hydroxytryptophan

207,551

0.0021

Melatonin

677,434

< 0.001

5-HIAA

> 2,000,000

< 0.001

L-Tryptophan

> 20,000,000

< 0.0001

 


Recent Citations:

Leung, Joseph, et al. “Salivary Serotonin Does Not Correlate with Central Serotonin Turnover in Adult Phenylketonuria (PKU) Patients.” Molecular Genetics and Metabolism Reports, vol. 15, 1 June 2018, pp. 100–105., doi:10.1016/j.ymgmr.2018.03.008.

Saraf, MK;Piccolo, BD;Bowlin, AK;Mercer, KE;LeRoith, T;Chintapalli, SV;Shankar, K;Badger, TM;Yeruva, L;, (2017). Formula diet driven microbiota shifts tryptophan metabolism from serotonin to tryptamine in neonatal porcine colon. Microbiome, 5(1), 77-

Utilizing Mouse/Rat Samples:

  • Malkova, NV;Gallagher, JJ;Yu, CZ;Jacobs, RE;Patterson, PH; Manganese-enhanced magnetic resonance imaging reveals increased DOI-induced brain activity in a mouse model of schizophrenia, Proc. Natl. Acad. Sci. U.S.A., 2014, Volume 111, Issue 24, Page E2492-500

 

 Find Similar Products by Category

Vendors Other Products

Eagle Biosciences, Inc.
20A NW Blvd., Suite 112
Nashua, NH 03063
Email: info@eaglebio.com
Toll Free: 866-411-8023
International: +617-419-2019

Click the button below to add the Serotonin Ultrasensitive ELISA Assay Kit to your wish list.