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SDMA (Symmetric Dimethylarginine) ELISA Assay Kit

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$960.00
SKU:
SDM31-K01

 Product Description

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SDMA (Symmetric Dimethylarginine) ELISA Assay kit: 
For Research Use Only
Size:  1x96 wells
Sensitivity:  0.03 µmol/L
Dynamic Range:  0.08 - 3.0  µmol/L
Incubation Time:  3 hours
Sample Type:  Serum, Plasma   
Sample Size: 20 µL

Controls Included 
Product Developed and Manufactured in Germany EA203/96

Reference Values (Normal)
0.3 – 0.75 µmol/l (60 – 150 ng/ml)

It is suggested that each laboratory establish its own normal ranges

Intended Use
The Eagle Biosciences SDMA (Symmetric Dimethylarginine) ELISA Assay Kit is intended for the quantitative determination of SDMA (Symmetric Dimethylarginine) in serum or plasma. The SDMA (Symmetric Dimethylarginine) ELISA Assay Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures. 
Human, Canine, and Feline SDMA can be detected using this SDMA (Symmetric Dimethylarginine) ELISA Assay Kit.

Assay Principle
The competitive SDMA (Symmetric Dimethylarginine) ELISA Assay Kit uses the microtiter plate format.  SDMA is bound to the solid phase of the microtiter plate. SDMA in the samples is acylated and competes with solid phase bound SDMA for a fixed number of rabbit anti-SDMA antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase SDMA is detected by anti-rabbit / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase SDMA is inversely proportional to the SDMA concentration of the sample.

Assay Background

The Eagle Biosciences SDMA (Symmetric Dimethylarginine) ELISA Assay Kit is intended for the quantitative determination of SDMA (Symmetric Dimethylarginine) in serum or plasma. The SDMA (Symmetric Dimethylarginine) ELISA Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures. 

Dosing of most drugs must be adapted in renal insufficiency, making accurate assessment of renal function an essential component of diagnostics in clinical medicine. Furthermore, even modest impairment of renal function has been recognized as a cardiovascular risk factor. As the most commonly used marker of renal excretory function, serum creatinine concentration, does not adequately respond to mild to moderate impairment of renal function, more sensitive markers for renal excretory function are urgently sought, especially in mild stages of renal impairment. SDMA is a methylated derivative of the amino acid L-arginine (symmetric dimethylarginine). SDMA is eliminated from the body exclusively by renal excretion; therefore SDMA plasma concentration is tightly related to renal function. Thus, quantification of plasma SDMA is an adequate means to assess renal function, as could be demonstrated in a series of recent clinical trials: In 18 clinical studies involving more than 2,100 samples systemic SDMA concentrations were highly correlated with inulin clearance as well as with various clearance estimates and better corresponded to mild renal function impairment than serum creatinine.

Thus, SDMA exhibits properties of a reliable marker of renal function. Furthermore, there is evidence showing that elevated SDMA levels, as they may occur in renal function impairment, may prospectively indicate future risk of cardiovascular disease and mortality independently of the level of renal impairment.

Typical Standard Curve:

 

SDMA and Chronic Kidney Disease (CKD)

SDMA ELISA is suitable for an early detection of canine and feline chronic kidney disease.  Research has shown that SDMA can identify CKD an average of 9 months earlier in dogs and 17 months sooner in cats—in one cat 4 years earlier than traditional biomarkers.
 
SDMA is a methylated form of the amino acid arginine, which is produced in every cell and released into the body’s circulation during protein degradation. SDMA is excreted almost exclusively by the kidneys, making it a good marker for estimating kidney function.  In addition, SDMA is not impacted by muscle mass, thereby providing practitioners a better tool for diagnosing and monitoring CKD in thin geriatric animals, especially cats and animals with other diseases that cause muscle wasting.
 
The SDMA ELISA is partially automatable and suitable for small sample volumes (20 µl). It offers very good specificity, sensitivity, excellent linearity and reproduction. The ELISA correlates perfectly with the SDMA-determination by HPLC and LC-MS/MS.

Recent Citations:

El-Sadek, AE;Behery, EG;Azab, AA;Kamal, NM;Salama, MA;Abdulghany, WE;Abdallah, EA; Arginine dimethylation products in pediatric patients with chronic kidney disease. Annals of Medicine and Surgery. 2016: 9(8): 22-27.

 

 

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Eagle Biosciences, Inc.
20A NW Blvd., Suite 112
Nashua, NH 03063
Email: info@eaglebio.com
Toll Free: 866-411-8023
International: +617-419-2019

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