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S-Adenosyl-L-homocysteine (SAH) ELISA Assay Kit

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$1,395.00
SKU:
SAH31-K01

 Product Description

Package insert view pdf

 

 

S-Adenosyl-L-homocysteine (SAH) ELISA Assay Kit:
For Research Use Only
Size:  1x96 wells
Sensitivity:  31.25 nM
Dynamic Range:  31.25 - 1000 nM
Incubation Time:  1.5 hours
Sample Type:  Serum, Plasma
Sample Size: 50 µL
Control Included

 

Intended Use
The Eagle Biosciences S-Adenosyl-L-homocysteine (SAH) ELISA Assay Kit is intended for the quantitative determination of S-Adenosyl-L-homocysteine (SAH) in serum or plasma.  The Eagle Biosciences S-Adenosyl-L-homocysteine (SAH) ELISA Assay Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.

Assay Background

Methionine is an amino acid with sulfur methyl group, which is converted into S-adenosylmethionine (SAM) with ATP by methionine adenosyltransferase (EC 2.5.1.6). SAM is the sole methyl group donor for over 50 kinds of biologically active substances such as DNA, RNA, protein, phospholipids, hormones and neuro-transmitters, etc. After methyl group is transferred away from SAM under a mehtyltransferase, S-adenosylhomocysteine is formed, which is further metabolized into homocysteine (Hcy) after adenosine is removed. The homocysteine ​​is metabolized to methionine through N5-methyl tetrahydrofolate methyltransferase (EC1.1.1.68) and coenzyme vitamin B12 catalytic pathway accepting methyl from N5-methyltetrahydrofolate. This is methionine cycle.

Methylation index is defined as the ratio of SAM and SAH. Methylation index is a better marker for methylation status and methylation capability.  SAH and homocysteine connect the processes of providing methyl group to critical biological molecules and recycling methyl group from N-5-methyltetrahydrofolate. The levels of SAH will determine or reflect whether the methionine cycle is normal or not. Therefore, finding a way to better quantify them has valuable practical implications. Elevated SAH can cause damage to vascular endothelial cells, which is related to the levels of global genome methylation. SAH may be a potential biomarker for atherosclerosis.  This S-Adenosyl-L-homocysteine (SAH) ELISA Assay Kit is used to measure the level of SAH in serum or plasma of soluble form.

Principle of the Assay

This direct competitive S-Adenosyl-L-homocysteine (SAH) ELISA Assay Kit (Enzyme Linked Immunosorbent assay) is designed to measure the level of S-adenosyl-L-methionine (SAH) in the sample. SAH conjugated with macromolecule is immobilized on the micro-titer plate. Standards and samples are pipetted into the wells, and then the HRP-conjugated antibody against SAH is added. The free SAH molecule in samples or standards competes with the immobilized SAH on the micro-titer plate surface for binding sites of the antibody. After discarding the mixed solution and washing each well, TMB substrate solution is added. The substrate solution turns blue under the effect of HRP (horseradish peroxidase), and changes into yellow once stop solution (acid) is added. The color develops in inverse proportion to the amount of SAH in the sample (or standards).  The optical density of the remaining solution (OD450) is measured at 450nm using micro-plate spectrophotometer. The level of SAH in samples can be calculated through standard curve generated with standards.

 

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Eagle Biosciences, Inc.
20A NW Blvd., Suite 112
Nashua, NH 03063
Email: info@eaglebio.com
Toll Free: 866-411-8023
International: +617-419-2019

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