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Rotavirus Antigen ELISA Assay Kit

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$500.00
SKU:
RTV35-K01

 Product Description

Package insert view pdf
 

 


Rotavirus Antigen ELISA Assay Kit:
For Research Use Only
Size:  1x96 wells
Sensitivity:  Cut-Off Control
Incubation Time:  2 hours
Sample Type:  Stool
Sample Size: 90mg

Intended Use
Eagle Biosciences Fecal Rotavirus Antigen ELISA (enzyme linked immunosorbent assay) kit is intended for the qualitative and quantitative detection of rotavirus antigen in feces. Specifically, this test detects VP6 of the viral capsid and is useful in detection of type A rotavirus infection.  The assay is a useful tool in the diagnosis of active rotavirus infection in acute or chronic gastroenteritis. The Eagle Biosciences Fecal Rotavirus Antigen ELISA Assay kit is intended for research use only and not intended for diagnostic procedures.

Background
Rotaviruses are the main and the most important pathogens that cause of non-bacterial acute gastroenteritis and diarrhea, especially in children from 6 months to 2 years of age, premature infants, the elderly, and the immunocompromised individuals. Rotaviruses have been identified in almost 40% of the faces of children with gastroenteritis. Rotavirus is the cause of up to 50% of the hospitalized cases of diarrhea in infant and young children. Almost every child has been infected with rotavirus by age 5. Over 3 million cases of rotavirus gastroenteritis occur annually in the US. There are about 120 million rotavirus infections every year worldwide and that causes the death of 600,000 to 650,000 children.  Study also indicates that a high frequency of rotavirus infections may increase the risk of celiac disease autoimmunity in childhood in genetically predisposed individuals.

Rotaviruses have a genome consisting of 11 double-stranded RNA segments surrounded by a distinctive three-layered icosahedral protein capsid. The first layer is formed by the protein VP2, with each vertex having a copy of the proteins VP1 and VP3. The second layer is formed by the protein VP6. The outermost protein layer is composed of the structural glycoprotein VP7 and the spike protein VP4. Viral particles are up to 100 nm in diameter and have a buoyant density of 1.36 g/ml in CsCl. Rotaviruses tend to affect gastrointestinal epithelial cells that are at the tip of the villus. Their triple protein coats make them very resistant to the normally prohibitive pH of the stomach, and also digestive enzymes (lipases and proteases) in the gastrointestinal tract. During the infection, rotavirus produces mRNA to support both protein translation and genome replication.

Rotavirus is transmitted by oral-fecal contact with an incubation period of 1-3 days. Characteristic symptoms include vomiting, hydrodiarrhoea for between 3 and 8 days, high temperature and stomach pains. A large amount of rotavirus particles is shed during infection.

Specific diagnosis of the rotavirus infection is made by identification of the virus in the patient's stool. Enzyme linked immunsorbent assay (ELISA) is the test most widely used to screen clinical specimens. Electron microscopy and polyacrylamide gel electrophoresis are used in some laboratories in addition or as an alternative to ELISA.

Assay Principle

This Eagle Biosciences Fecal Rotavirus Antigen ELISA is designed, developed and produced for the qualitative and quantitative measurement of rotavirus antigen in test specimen. The assay utilizes the microplate-based enzyme immunoassay technique by coating highly purified antibody onto the wall of microtiter well.
Assay controls and fecal specimen, as well as HRP-conjugated monoclonal antibody that specifically recognize the inner capsid protein of the rotaviruses are added to microtiter wells of microplate that was coated with a highly purified polyclonal anti-rotavirus antibody on its wall. After an incubation period an immunocomplex of “Anti-Rotavirus Antibody – Rotavirus Antigen – HRP-conjugated Anti-rotavirus Tracer Antibody” was formed if there is rotavirus antigen present in the test sample. The unbound tracer antibody and other protein or buffer matrix are removed in the subsequent washing step. HRP-conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to rotavirus captured on the wall of each microtiter well is directly proportional to the amount of rotavirus antigen level in each test specimen.

Standard Curve

 

REFERENCES

  1. Set-up of a new rapid immunochromatographic diagnostic test for a Rotavirus detection. D. Van Beers , M. DE Foor , R. Viehoff , D. Col , M. Venuti and T. Leclipteux.Progress in Clinical Virology III , Bologne , Septembre 1997.
  2. Detection of rotavirus in faecal specimens with a monoclonal antibody enzyme-linked immunosorbent assay: comparison with polyclonal antibody enzyme-immunoessays and a latex agglutination test. Sneyers et al.Comp. Immun. Microbiol. Infect. Dis. , vol 12 , n°4 , pp 95-104 , 1989
  3. Comparison of Three Rapid Immunoassays for the Detectiono of Rotavirus Antigen in Stool Samples I. Van der Donck et al. ESCV Winter Meeting 1999, Rotterdam, the Netherlands
  4. Evaluacion de tres Metodos de Deteccion de Rotavirus en Heces I. Wilhelmi et al. 6th Congresso Nacional de Virologia, Madrid, 26th Oct. 99

 

 

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Eagle Biosciences, Inc.
20A NW Blvd., Suite 112
Nashua, NH 03063
Email: info@eaglebio.com
Toll Free: 866-411-8023
International: +617-419-2019

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