Loading... Please wait...

MutaGEL® MTHFR PCR Assay

Hover over image to zoom

$750.00
SKU:
KT0001

 Product Description

Package insert view pdf

 

 

MutaGEL® MTHFR PCR Assay Kit
For Research Use Only
Size:  24 Samples
Method:  PCR (allel specific)
Sample Type:  DNA (e.g.whole blood, cheek swab) 
Sample Size: 200 µL

Product Developed and Manufactured by Immundiagnostik


Intended Use
The Eagle Biosciences MutaGEL® MTHFR kit allows the detection of the most common C677T polymorphism of the human MTHFR gene encoding for the enzyme 5,10-methylene-tetrahydrofolatereductase. This mutation causes the substitution of alanine with valine within the enzyme (Ala 222 Val). The Eagle Biosciences MutaGEL® MTHFR is for research use only and is not intended for diagnostic or therapeutic procedures. The Eagle Biosciences MutaGEL® MTHFR is for research use only and is not intended for diagnostic or therapeutic procedures.

Assay Principle

The Eagle Biosciences MutaGEL® MTHFR PCR Assay Kit contains a set of primers which amplify a specific sequence within the human MTHFR gene. The amplified product obtained from a wild type DNA will not be cut by the restriction enzyme included in this kit, whereas the fragment obtained from DNA carrying the very common C677T- mutation will be cut once. The generated amplification products and their cut-fragments are analyzed through gel electrophoresis.

 

Assay Background

Hyperhomocysteinemia is the result of a disturbed homocysteine metabolism often due to genetic defects. The increase of homocysteine plasma level is therefore a risk factor for cardio- or cerebrovascular complications as well as for venous thrombosis and mirgaine. Patients often carry the very common C677T mutation in the MTHFR gene coding for a thermolabile variant of the MTHFR enzyme with reduced activity. Also an A1298C mutation in the MTHFR gene increases the homocysteine plasma level additionally, but only in case of already present C677T heterozygotie. Nevertheless, it could be an option to analyse also this polymorphism in a separate PCR to determine complete genetic risk factors for hyperhomocysteinemia.

 Find Similar Products by Category

Vendors Other Products

Eagle Biosciences, Inc.
20A NW Blvd., Suite 112
Nashua, NH 03063
Email: info@eaglebio.com
Toll Free: 866-411-8023
International: +617-419-2019

Click the button below to add the MutaGEL® MTHFR PCR Assay to your wish list.