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MANF ELISA Assay Kit

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$990.00
SKU:
MNA31-K01

 Product Description

Package insert view pdf

 

 

MANF ELISA Assay kit:
For Research Use Only
Size:  1 x 96 wells
Sensitivity:  0.25 pg/ml
Dynamic Range:  30 - 75 pg/mL
Incubation Time:  2.5 hours
Sample Type:  Serum, Plasma, Cell Culture Supernatant
Sample Size: 100 µL

Intended Use
The Eagle Biosciences MANF ELISA Assay Kit is intended for the quantitative determination of human MANF in serum, plasma, and cell culture supernatant. The MANF ELISA Assay Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.

Assay Principle
Eagle Biosciences human MANF Assay kit is based on the quantitative sandwich enzyme immunoassay technique. Microtiter wells are pre-coated with human MANF-specific monoclonal capture antibodies. Samples and standards are pipetted into microwells and human MANF molecules present in the sample are bound by the capture antibodies. After incubation, unbound material is removed by washing the wells. Then, horseradish peroxidase (HRP) conjugated human MANF-specific polyclonal detection antibodies bind to different epitopes of human MANF molecules. After washing, the ready to use HRP substrate (TMB) is added to wells. The intensity of the colour produced is directly proportional to the amount of human MANF in the sample. Colour development is then stopped by the addition of stop solution. Absorbance is measured at 450 nm.

  1. Dilute 50 mL of wash concentrate with 450 mL of distilled water to prepare washing solution.
  2. Perform dilutions of each sample in sample diluent (pink).
  3. Add 100 μL of samples and standards into appropriate wells in duplicate.
  4. Incubate the covered microplate for 60 min at RT on a microwell plate shaker (300 rpm).
  5. Prepare enzyme conjugate working solution by diluting enzyme conjugate stock in enzyme conjugate stock diluent (blue) (1:100)
  6. Discard the solution and wash the wells 4 times with 300 μL of washing solution.
  7. Add 100 μL of enzyme conjugate working solution into each well.
  8. Incubate the covered microplate for 60 min at RT on a microwell plate shaker (300 rpm).
  9. Discard the solution and wash the wells 4 times with 300 μL of washing solution.
  10. Add 100 μL of substrate solution into each well.
  11. Incubate the covered microplate for 20 minutes at RT on a microwell plate shaker (300 rpm).
  12. Stop the reaction by adding 50 μl of STOP solution into each well in the same order and time as for TMB distribution.
  13. Read the absorbance at 450 nm immediately.

Typical Standard Curve

 

 

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Eagle Biosciences, Inc.
20A NW Blvd., Suite 112
Nashua, NH 03063
Email: info@eaglebio.com
Toll Free: 866-411-8023
International: +617-419-2019

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