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H. pylori Antigen Quantitative ELISA Assay Kit

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$440.00
SKU:
HPL35-K01

 Product Description

Package insert view pdf
 

 


Helicobacter pylori Antigen Quantitative ELISA Assay Kit:
For Research Use Only
Size:  1x96 wells
Sensitivity:  0.165 ng/mL
Dynamic Range:  0.19 - 150 ng/mL
Incubation Time:  2 hours

Sample Type:  Stool


Intended Use
Eagle Biosciences Helicobacter pylori Antigen ELISA Assay Kit is intended for use in the quantitative determination of H. pylori antigen in fecal samples.  The H. pylori ELISA Assay Kit is for research use only and not intended for diagnostic procedures.. 


Background
H. pylori (previously known as Campylobacter pyloridis) is a type of bacteria that infects the stomach and is a common cause of peptic ulcers. H. pylori bacteria can be passed from person to person through direct contact with saliva, vomit or fecal matter. H. pylori can also be spread through contaminated food or water.  The infection is normally acquired during childhood. H. pylori usually goes undiagnosed until symptoms of a peptic ulcer occur. H. pylori infection is quite common and is present in about half the people in the world.

Assay Principle

This Helicobacter pylori Antigen ELISA Assay Kit “sandwich” ELISA is designed, developed and produced for the quantitative measurement of H. pylori antigen in stool specimen. The assay utilizes the microplate-based enzyme immunoassay technique by coating highly purified antibody onto the wall of microtiter wells.

Assay calibrators and fecal specimen are added to microtiter wells of microplate that was coated with a highly purified monoclonal H. pylori antibody on its wall. During the assay, the H. pylori antigen will be bound to the antibody coated plate after an incubation period. The unbound material is washed away and another HRP-conjugated monoclonal antibody which specifically recognizes the protein of H. pylori is added for further immunoreactions.  After an incubation period, the immunocomplex of “H. pylori Antibody – H. pylori Antigen – HRP-conjugated Anti-H. pylori Tracer Antibody” is formed if H. pylori antigen is present in the test sample. The unbound tracer antibody and other proteins in buffer matrix are removed in the subsequent washing step. HRP conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to H. pylori proteins captured on the wall of each microtiter well is directly proportional to the amount of H. pylori antigen level in each test specimen.

Calibration Curve

Specificity

The Fecal H. pylori Ag ELISA Assay does not cross react to the following organisms: Cryptosoridium parvum, Giardia lamblia, rotavirus and adenovirus.

 

REFERENCES

  1. Ungar BL, Yolken RH, Nash TE, Quinn TC. Enzyme-linked immunosorbent assay for the detection of H. Pylori in fecal specimens. J Infect Dis. 1984 Jan;149(1):90-7.
  2. Rosenblatt JE, Sloan LM, Schneider SK. Evaluation of an enzyme-linked immunosorbent assay for the detection of H. Pylori in stool specimens. Diagn Microbiol Infect Dis. 1993 May-Jun;16(4):337-41.
  3. Stibbs HH, Samadpour M, Manning JF.       Enzyme immunoassay for detection of H. Pylori cyst antigens in formalin-fixed and unfixed human stool. J Clin Microbiol. 1988 Sep;26(9):1665-9.
  4. Stibbs HH. Monoclonal antibody-based enzyme immunoassay for H. Pylori antigen in human stool. J Clin Microbiol. 1989 Nov;27(11):2582-8.
  5. Janoff EN, Smith PD, Blaser MJ. Acute antibody responses to H. Pylori are depressed in patients with AIDS. J Infect Dis. 1988 Apr;157(4):798-804.

 

 

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Eagle Biosciences, Inc.
20A NW Blvd., Suite 112
Nashua, NH 03063
Email: info@eaglebio.com
Toll Free: 866-411-8023
International: +617-419-2019

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