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Dopamine Sensitive ELISA

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$870.00
SKU:
DOU39-K01

 Product Description

Package insert view pdf

 

 

Dopamine Sensitive ELISA Assay kit:
For Research Use Only
Size:  1x96 wells
Sensitivity:  5.9 pg/ml
Dynamic Range:  0.3 - 100 ng/ml
Incubation Time:  Overnight
Sample Type:  Biological Samples
Sample Size: 20 µl
Controls Included

Product Developed and Manufactured in Germany EA634/96

Conversion into pmol/l:
Dopamine: 1 pg / ml = 6,53 pmol / l

Intended Use
The Dopamine High Sensitive ELISA Assay kit is an Enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of dopamine in biological samples including serum, plasma, tissue, and cell culture samples.  The Dopamine High Sensitive ELISA Assay kit is for research use only and should not be used in diagnostic procedures.
 
Assay Principle
The competitive Dopamine High Sensitive ELISA kit uses the microtiter plate format. Dopamine is bound to the solid phase of the microtiter plate. Acylated catecholamine from the sample and solid phase bound catecholamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase catecholamine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample.
Pipette each 100 µl prepared Standards, Controls and Samples into the respective wells (colour coded green).

  1. Pipette each 20 µl Dopamine-Antiserum (colour coded green) into all wells).
  2. Cover the plate with adhesive foil, shake briefly and incubate for 15 – 20 hours (overnight) at 2 - 6 °C.
  3. Discard or aspirate the contents of the wells and wash thoroughly with each 250 µl prepared Wash Buffer. Remove residual liquid by tapping the inverted plate on clean absorbent paper. Repeat the washing procedure 3 times.
  4. Pipette each 100 µl POD-Conjugate into all wells.
  5. Incubate for 60 minutes at room temperature on an orbital shaker (medium shaking rate).
  6. Washing: Repeat wash step 4.
  7. Pipette each 100 µl Substrate into all wells.
  8. Incubate for 35 to 45 minutes at room temperature (20 - 25°C) on an orbital shaker (medium shaking rate). Avoid exposure to direct sun light.
  9. Pipette 100 µl Stop Solution into all wells.
  10. Read the optical density at 450 nm (reference wavelength between 570 and 650 nm) in a microplate photometer within 15 minutes.

Assay Background

The Dopamine High Sensitive ELISA Assay kit is an Enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of dopamine in biological samples including serum, plasma, tissue, and cell culture samples.  The Dopamine High Sensitive ELISA Assay kit is for research use only and should not be used in diagnostic procedures.  Catecholamine is the name of a group of aromatic amines (noradrenaline, adrenaline, dopamine, and their derivatives) which act as hormones and neurotransmitter, respectively. Adrenaline and noradrenaline are formed from dopamine. They act on the cardiac musculature and the metabolism (adrenaline) as well as on the peripheral circulation (noradrenaline) and help the body to cope with acute and chronic stress.

An increased production of catecholamines can be found with tumours of the chromaffine system (pheochromocytoma, neuroblastoma, ganglio­neuroma). An increased or decreased concentration of the catechol­amines can also be found with hypertension, degenerative cardiac diseases, schizophrenia and manic-depressive psychosis. The Dopamine High Sensitive ELISA assay kit provides materials for the quantitative measurement of dopamine in low concentrated samples and for small sample volumes. Dopamine is extracted using a cis-diol-specific affinity gel and acylated to N-acyldopamine and then converted enzymatically into N-acyl-3-methoxytyramine.

Typical Standard Curve

Standard

A

B

C

D

E

F

Dopamine (ng/ml)

0

0.3

1.25

5

20

100

Dopamine (nmol/l)

0

2

8.2

32.7

131

653

 

 

Recent Citations

 

Wakeman, Dustin R., Benjamin M. Hiller, David J. Marmion, Christopher W. Mcmahon, Grant T. Corbett, Kile P. Mangan, Junyi Ma, Lauren E. Little, Zhong Xie, Tamara Perez-Rosello, Jaime N. Guzman, D. James Surmeier, and Jeffrey H. Kordower. "Cryopreservation Maintains Functionality of Human iPSC Dopamine Neurons and Rescues Parkinsonian Phenotypes In Vivo." Stem Cell Reports9.1 (2017): 149-61. Web.

Lantz, SM;Cuevas, E;Robinson, BL;Paule, MG;Ali, SF;Imam, SZ; The Role of Harmane and Norharmane in In Vitro Dopaminergic Function, Journal of Drug and Alcohol Research, 2015.

Hood, RL;Liguore, WA;Moore, C;Pflibsen, L;Meshul, CK; Exercise intervention increases spontaneous locomotion but fails to attenuate dopaminergic system loss in a progressive MPTP model in aged mice, Brain Res., 2016. DOI: 10.1016/j.brainres.2016.06.032

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Nashua, NH 03063
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International: +617-419-2019

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