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Dopamine ELISA Assay Kit

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$520.00
SKU:
DOP31-K01

 Product Description

Package insert view pdf

 

 

Dopamine ELISA Assay kit:
For Research Use Only
Size:  1x96 wells
Sensitivity:  Urine:  0.44 ng/ml, Plasma:  29 pg/ml
Dynamic Range:  1.5 - 2560 ng/ml (9.8 - 16717 nmol/L)
Incubation Time:  Overnight
Sample Type:  Urine, Plasma
Sample Size: 15 µL (Urine), 300 µl (Plasma)
Controls Included

Product Developed and Manufactured in Germany EA608/96

Reference Values (Normal)
Urine    < 600 µg/day      
Plasma  < 100 pg/ml

It is suggested that each laboratory establish its own normal ranges

Intended Use
The Eagle Biosciences Dopamine ELISA Assay Kit is intended for the quantitative determination of Dopamine in urine or plasma. The Dopamine ELISA
Assay Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures. 

Assay Principle
The competitive Dopamine ELISA
Assay kit uses the microtiter plate format. Dopamine is bound to the solid phase of the microtiter plate. Acylated dopamine from the sample and solid phase bound dopamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase dopamine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase dopamine is inversely proportional to the dopamine concentration of the sample.

Assay Background

The Eagle Biosciences Dopamine ELISA Assay Kit is intended for the quantitative determination of Dopamine in urine or plasma. The Dopamine ELISA Assay Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.  Catecholamine is the name of a group of aromatic amines (noradrenaline, adrenaline, dopamine, and their derivatives) which act as hormones and neurotransmitter, respectively. Adrenaline and noradrenaline are formed from dopamine. They act on the cardiac musculature and the metabolism (adrenaline) as well as on the peripheral circulation (noradrenaline) and help the body to cope with acute and chronic stress.

An increased production of catecholamines can be found with tumors of the chromaffine system (pheochromocytoma, neuroblastoma, ganglio­neuroma). An increased or decreased concentration of the catechol­amines can also be found with hypertension, degenerative cardiac diseases, schizophrenia and manic-depressive psychosis. The Dopamine ELISA assay kit provides materials for the quantitative measurement of dopamine in plasma and urine. Dopamine is extracted using a cis-diol-specific affinity gel and acylated to N-acyl-dopamine and then converted enzymatically into N-acyl-3-methoxytyramine.

Typical Standard Curve

Cross Reactivity Data

Components

Cross Reactivity (%)

Dopamine-Ab

Adrenaline

< 0.020

Noradrenaline

0.23

Dopamine

100

Metanephrine

< 0.020

Normetanephrine

< 0.020

3-Methoxytyramine

0.28

L-Dopa

< 0.01

Tyramine

0.011

Tyrosine

< 0.01

Homovanillic acid (HVA)

< 0.01

Vanillylmandelic acid (VMA)

< 0.01

 

Recent Citations

Yadav, Gaya, et al. “Novel Organelle Anion Channels Formed by Chromogranin B Drive Normal Granule Maturation in Endocrine Cells.” BioRxiv, 17 Apr. 2018, doi:10.1101/302828.Kandil, EA;Abdelkader, NF;El-Sayeh, BM;Saleh, S;, (2016). Imipramine and amitriptyline ameliorate the rotenone model of Parkinson's disease in rats, 33226-37.

Abu Ghazaleh, H;Lalies, MD;Nutt, DJ;Hudson, AL; The modulatory action of harmane on serotonergic neurotransmission in rat brain., Brain Res.;2015, p57-64, Vol. 1597. DOI: 10.1016/j.brainres.2014.11.056

Using Tissue/Cell Culture Samples:

Simpson, N;Maffei, A;Freeby, M;Burroughs, S;Freyberg, Z;Javitch, J;Leibel, RL;Harris, PE, Dopamine-mediated autocrine inhibitory circuit regulating human insulin secretion in vitro, Mol. Endocrinol., 2012, V. 26, Page , 1757-72, http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3458225/

 

 

 

 

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Eagle Biosciences, Inc.
20A NW Blvd., Suite 112
Nashua, NH 03063
Email: info@eaglebio.com
Toll Free: 866-411-8023
International: +617-419-2019

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