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Now Available: Human Anti-IgE Antibody ELISA!

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Human Anti-IgE Antibody ELISA Assay Kit

This Eagle Biosciences Human Anti-IgE Antibody ELISA Assay Kit is produced for the quantitative determination of human anti-human IgE antibody levels in human serum or plasma samples. The test may be useful for detecting patients who have developed antibodies (mainly IgG) to their own IgE. This kit is for research purposes only. It is not for use in diagnostic procedures.

Size: 1x96 wells
Sensitivity: 0.4808 ng/mL
Dynamic Range: 12 - 450 ng/mL
Incubation Time: >3 hours
Sample Type: Serum or Plasma
Sample Size: 0.2 mL

SHORT ASSAY PROTOCOL

  1. Add 100 μL of calibrators, control and patient sample to the plate
  2. Incubate 1 hour at RT, shaking
  3. Wash strips with diluted wash buffer
  4. Add 100 μL biotin Antibody
  5. Incubate 30 min at RT, shaking
  6. Wash strips with diluted wash buffer
  7. Add 100 μL Streptavidin-HRP
  8. Incubate 30 min at RT, shaking
  9. Wash strips with diluted wash buffer
  10. Add 100 μL TMB substrate
  11. Incubate 20 min at RT
  12. Add 100 μL stop solution
  13. Read strips at OD 450 nm

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Nashua, NH 03063
Email: info@eaglebio.com
Toll Free: 866-411-8023
International: +617-419-2019