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Adenovirus Antigen ELISA Assay Kit

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$500.00
SKU:
ADV35-K01

 Product Description

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Adenovirus Antigen ELISA Assay Kit:
For Research Use Only
Size:  1x96 wells
Sensitivity:  Cut-Off Control
Incubation Time:  2 hours
Sample Type:  Stool
Sample Size: 50mg

Intended Use
This Eagle Biosciences Fecal Adenovirus Antigen ELISA Assay Kit is a microplate-based ELISA (enzyme linked immunosorbent assay) kit is intended for the qualitative detection of Adenovirus antigen in feces. The assay is a useful tool in the diagnosis of active Adenovirus infection in acute or chronic gastroenteritis. This Eagle Biosciences Fecal Adenovirus Antigen ELISA Assay Kit is for Research Use Only and is not intended for diagnostic or therapeutic purposes.

Background

Acute diarrheal disease in young children is a major cause of morbidity worldwide and is a leading cause of mortality in developing countries. Research has shown that enteric adenoviruses, primarily Ad40 and Ad41, are a leading cause of diarrhea in many of these children, second only to the rotaviruses. However many different symptoms can manifest, depending on the type of infecting Adenovirus. There are 49 distinct serotypes that can cause infections in humans.

The diarrhea resulting from enteric adenoviruses is longer in duration than that caused by the rotaviruses, usually lasting 7 - 8 days. Adenovirus infections often show up as conjunctivitis, tonsillitis (which may look exactly like strep throat and cannot be distinguished from strep except by throat culture), an ear infection, or croup. Adenoviruses can also cause gastroenteritis (stomach flu). A combination of conjunctivitis and tonsillitis is particularly common with adenovirus infections. Small children are especially prone to develop adenovirus bronchiolitis or pneumonia, both of which can be severe. In babies, adenoviruses can also cause coughing fits that are almost exactly like whooping cough. Adenoviruses can also lead to viral meningitis or encephalitis. Rarely, adenovirus causes inflammation of the urinary bladder (also known as cystitis), producing blood in the urine. In children, adenoviruses may cause acute upper respiratory infections with fever and runny nose. Adenovirus types 1, 2, 3, 5, and 6 are responsible for most of these infections.

Specific diagnosis of the Adenovirus infection is made by identification of the virus in the patient's stool. Enzyme linked immunsorbent assay (ELISA) is the test most widely used to screen clinical specimens. Electron microscopy and polyacrylamide gel electrophoresis are used in some laboratories in addition or as an alternative to ELISA.

Assay Principle

This Eagle Biosciences Fecal Adenovirus Antigen ELISA Assay Kit is designed, developed and produced for the qualitative measurement of Adenovirus antigen in test specimen. The assay utilizes the microplate-based enzyme immunoassay technique by coating highly purified antibody onto the wall of microtiter well.

Assay controls and fecal specimen, as well as HRP-conjugated monoclonal antibody that specifically recognizes the inner capsid protein of the Adenoviruses are added to microtiter wells of microplate that was coated with a highly purified polyclonal anti-Adenovirus antibody on its wall. After an incubation period an immunocomplex of “Anti-Adenovirus Antibody – Adenovirus Antigen – HRP-conjugated Anti-Adenovirus Tracer Antibody” is formed if there is Adenovirus antigen present in the test sample. The unbound tracer antibody and other protein or buffer matrix are removed in the subsequent washing step. HRP-conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to Adenovirus captured on the wall of each microtiter well is directly proportional to the amount of Adenovirus antigen level in each test specimen.

 

REFERENCES

  1. Edwards KM, Thompson J, Paolini J, Wright PF.  Adenovirus infections in young children. Pediatrics. 1985 Sep;76(3):420-424
  2. Halonen P, Sarkkinen H, Artstila P, Hjertsson E, Torfason E. Four-layer radioimmunoassay for detection of adenovirus in stool. J Clin Microbiol.  1980 jun;11(6):614-617.
  3. Meurman O, Ruuskanen O, Sarkkinen H. Immunoassay diagnosis of adenovirus infections in children. J Clin Microbiol. 1983 Nov;18(5):1190-1195

 

 

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Eagle Biosciences, Inc.
20A NW Blvd., Suite 112
Nashua, NH 03063
Email: info@eaglebio.com
Toll Free: 866-411-8023
International: +617-419-2019

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