Steroid ELISA Assay

FSH ELISA Assay Kit

$215.00

The Eagle Biosciences Follicle Stimulating Hormone (hFSH) ELISA kit is for the quantitative determination of Follicle Stimulating Hormone human serum. The Follicle Stimulating Hormone ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

SKU: FSH31-K01 Categories: , ,

FSH ELISA Assay Kit

The FSH ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 1 IU/L
Dynamic Range: 5-100IU/L
Incubation Time: 1 hour 15 minutes
Sample Type: Serum
Sample Size: 25 µL

Controls Included

Assay Background

Human follicle stimulating hormone (FSH) is a glycoprotein hormone produced by the anterior pituitary gland. There are three other glycoprotein hormones, namely Thyroid Stimulating Hormone, Luteinizing Hormone (both produced by anterior pituitary gland) and Human Chorionic Gonadotropin (produced by the placenta) which are structurally similar. Each hormone has an alpha and beta subunit. The α subunits of each hormone are similar with the β subunit is specific to each hormone. The α subunits contain 92 amino acids with the β subunits vary with each hormone. The β subunit of both FSH and LH contain 115 amino acids, TSH 110 amino acids, and hCG 147 amino acids.
The FSH and LH hormones function differently in females and males. It is to be noted that in women the growth and maturation of the ovarian follicle is dependent of FSH, while in men both LH and FSH act on the testes.

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Additional Information

Assay Principle

“The principle of the following enzyme immunoassay test follows a typical two-step capture or
‘sandwich’ type assay. The assay makes use of two highly specific monoclonal antibodies: a monoclonal antibody specific for FSH is immobilized onto the microplate and another monoclonal antibody specific or a different region of FSH is conjugated to horse radish peroxidase (HRP). FSH from the sample and standards are allowed to bind to the plate, washed, and subsequently incubated with the HRP conjugate. After a second washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the color formed by the enzymatic reaction is directly proportional to the concentration of FSH in the sample. A set of standards is used to plot a standard curve from which the amount of FSH in samples and controls can be directly read.”

Documents

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